Human interleukin 2 (IL-2) ELISA detection kit operating instructions Detection principle The kit uses double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells pre-coated with the human interleukin 2 (IL-2) capture antibody, add the specimen, standard, and HRP-labeled detection antibody in sequence, incubate and wash thoroughly. The color is developed with the substrate TMB, which is converted into blue under the catalysis of peroxidase and into the final yellow under the action of acid. The color depth is positively correlated with human interleukin 2 (IL-2) in the sample. Measure the absorbance (OD value) at 450nm with a microplate reader to calculate the sample concentration. Sample collection, processing and storage methods 1. Serum: Use a test tube that does not contain pyrogens and endotoxins. Avoid any cell stimulation during the operation. After collecting blood, centrifuge at 3000 rpm for 10 minutes to quickly separate serum and red blood cells carefully. 2. Plasma: EDTA, citrate or heparin anticoagulation. Take the supernatant by centrifugation at 3000 rpm for 30 minutes. 3. Cell supernatant: centrifuge at 3000 rpm for 10 minutes to remove particles and polymers. 4. Tissue homogenate: Add tissue to the right amount of saline and mash. Take the supernatant by centrifugation at 3000 rpm for 10 minutes. 5. Preservation: If the sample is not tested in time after collection, please aliquot it in one dose and freeze it at -20 ℃ to avoid repeated freezing and thawing. Thaw at room temperature and ensure that the sample is thawed evenly and fully. Self-supplied items 1. Microplate reader (450nm) 2. High-precision sampler and pipette tip: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL Equilibrate at room temperature for 20 minutes at 2-8 ° C before use. The concentrated washing liquid taken out of the refrigerator will have crystals, which is a normal phenomenon. The water bath is heated to completely dissolve the crystals before use. 2. The slats not used in the experiment should be immediately returned to the ziplock bag, sealed (dry at low temperature) and stored. 3. The standard dilution can be regarded as a negative control or blank; the sample after pretreatment does not need to be diluted, just take 10μL and add it. 4. Perform the incubation operation strictly in accordance with the time, amount of liquid and sequence indicated in the manual. 5. Shake all liquid components thoroughly before use. Kit composition name 96-well configuration 48-well configuration Remarks Microwell microplate 12 wells × 8 wells 12 wells × 4 no standards (1200pg / ml) 0.6mL 0.6mL Dilute according to the instructions Standard dilution 6mL 3mL No samples Diluent 6mL 3mL No detection antibody-HRP 10mL 5mL No 20 × Wash buffer 25mL 15mL Dilute according to the instructions Substrate A 6mL 3mL No substrate B 6mL 3mL No stop solution 6mL 3mL No sealing film 2 sheets 2 sheets without instructions 1 One part, one part without ziplock bag, one part, no one. Note: Standards are diluted with standard dilutions in sequence as follows: 1200, 600, 300, 150, 75, 37.5 pg / ml Preparation of reagents 20 × dilution of washing buffer: distilled water Dilute 1:20, ie 1 part of 20 × washing buffer plus 19 parts of distilled water. Plate washing method 1. Manual plate washing: throw away the liquid in the hole, fill each hole with the washing liquid, let the liquid in the hole drain after standing for 1 min, pat dry on absorbent paper, and wash the plate 5 times in this way. 2. Automatic plate washing machine: Inject 350μL of washing liquid into each well, soak for 1min, and wash the plate 5 times. Operation steps 1. Take out the required slats from the aluminum foil bag after equilibrating at room temperature for 20min, and the remaining slats shall be sealed in a ziplock bag and put back at 4 ℃. 2. Set up standard wells and sample wells, add 50μL of standard products of different concentrations to the standard wells; 3. Add 10μL of the test samples to the test wells, and then add 40μL of the sample diluent; 4. Then the standard wells and samples 100 μL of detection antibody labeled with horseradish peroxidase (HRP) was added to each well, and the reaction well was sealed with a sealing plate membrane, and incubated for 60 min in a 37 ° C water bath or incubator. 5. Discard the liquid, pat dry on the absorbent paper, fill each well with the washing solution, let stand for 1min, shake off the washing solution, pat dry on the absorbent paper, and repeat washing the plate 5 times (you can also wash the plate with a washing machine). 6. Add 50 μL of substrate A and B to each well, and incubate at 37 ° C in the dark for 15 minutes. 7. Add 50μL of stop solution to each well, and measure the OD value of each well at 450nm wavelength within 15min. The result is judged to draw the standard curve: In the Excel worksheet, the standard product concentration is used as the abscissa, and the corresponding OD value is used as the vertical coordinate. Kit performance 1. Accuracy: The linear regression between the standard product and the expected concentration R value is greater than or equal to 0.9900. 2. Sensitivity: The minimum detection concentration is less than 1.0pg / ml. 3. Specificity: Does not cross-react with other soluble structural analogs. 4. Repeatability: The coefficients of variation within and between panels are less than 15%. 5. Storage: 2-8 ℃, protected from light and moisture. 6. Validity period: 6 months Disclaimer 1. The kit is for research use only and should not be used in clinical or human experiments, otherwise all consequences arising will be borne by the experimenter and the company will not be responsible. 2. Strictly follow the instructions, and the experimenter violates the instructions, and the consequences will be borne by the experimenter. Shanghai Yuping Biological Technology Co., Ltd. mainly deals with ELISA kits of various brands and grades, with quality assurance and perfect after-sales service. And provide free generation testing. Serving universities and immunology research units. Technicians serve you better.
Outdoor bench refers to the seating facilities used in outdoor places, often commonly found in parks, squares, leisure areas, scenic spots and other outdoor environments. The classification of outdoor benches can be introduced according to many aspects such as material, shape and function.
First, classification by material
1. Wooden bench: Wooden bench is one of the most common outdoor benches, usually made of natural wood, with natural and environmentally friendly characteristics. Wooden benches are usually made of solid wood or artificial wood, and the surface is treated with waterproof and anti-corrosion, which can adapt to various climatic conditions in the outdoor environment.
2. Metal bench: Metal bench is made of metal materials, such as cast iron, steel, etc. The metal bench is durable, corrosion resistant, easy to clean and is suitable for all kinds of outdoor environments.
3. Stone bench: Stone bench is made of marble, granite and other stone, which has the characteristics of good texture and beautiful appearance. Stone benches are generally suitable for high-end places such as garden scenic spots and can be integrated with the surrounding environment.
5. Combined material bench: Combined material bench refers to the bench made of different materials, such as the combination of wood and metal, stone and plastic combination. Combined material bench can usually combine the advantages of different materials, more in line with the needs of different outdoor environments.
2. Classification by shape
1. Linear bench: Linear bench is the most common shape of bench, generally composed of a row of seats, forming a straight line arrangement. The linear bench is suitable for outdoor places with large pedestrian flow and can provide more seats.
2. Arc bench: Arc bench is an arc bench composed of multiple seats, usually used in a ring seat area or landscape area. The curved bench can not only provide comfortable seating, but also increase the aesthetics of the place.
3. Wavy bench: The wavy bench is a bench composed of multiple seats in the shape of undulating waves, giving people a sense of movement and flow. The wavy bench is suitable for parks, squares and other places that need to increase vitality and fun.
4. Round bench: A round bench is a round bench composed of a number of seats, which is usually used in outdoor recreation areas and can provide a place for groups to gather.
3. Classification by function
1. Leisure bench: Leisure bench is a common form of outdoor bench, it can provide comfortable seats, so that people can relax in the outdoor environment.
2. Viewing bench: Viewing bench is usually set in a scenic outdoor environment, providing comfortable seats for people to enjoy the scenery.
3. Sitting bench: Sitting bench is usually set in parks, squares and other places with large traffic, to provide people with a short rest and relaxation.
4. Cool pavilion bench: Pavilion bench refers to a form of combination of bench and pavilion, which can provide sunshade, rain prevention and other functions, suitable for outdoor leisure areas.
In short, the classification of outdoor benches can be divided from many aspects such as material, shape and function. Different outdoor environments and needs need to choose a suitable bench type to provide comfortable and beautiful seating facilities
Beach Bench,Deck Chair,Deck Bench,Waterproof Bench
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