Experimental principle This kit uses the double antibody sandwich method to determine the level of rabbit tumor necrosis factor alpha (TNF-α) in the specimen. Microporous plates were coated with purified rabbit tumor necrosis factor alpha (TNF-α) antibody to make solid-phase antibodies. Tumor necrosis factor alpha (TNF-α) was added to the microwells coated with monoclonal antibody in turn, and then labeled with HRP The tumor necrosis factor α (TNF-α) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, and after thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the tumor necrosis factor alpha (TNF-α) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of rabbit tumor necrosis factor alpha (TNF-α) in the sample was calculated by a standard curve. For more detailed information about the indicators, please see: http: //
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