1. Carefully cut the strip containing the DNA to be recovered under the UV lamp, smash the cut strip (less than 0.6g) and place it in a 1.5ml centrifuge tube;
2. Add an equal volume of Tris-HCl saturated phenol (pH 7.6) and mix by shaking;
3. Place at -20 °C for 5-10 min;
4. Centrifuge at 10000g×5min at 4°C, transfer the upper layer to another centrifuge tube;
5. Add 1/4 volume of H2O to the gel-containing centrifuge tube and mix by shaking;
6. -20 ° C, placed 5-10min;
7. Centrifuge at 10000g × 5min at 4 ° C, and combine the supernatant;
8. Extract once with an equal volume of phenol/chloroform or chloroform and take the supernatant;
9. Add 1/10 volume of 3M NaAc (pH 5.2), 2.5 volumes of pre-cooled absolute ethanol, and mix;
10. -20 ° C, let stand for 30 min;
11. Centrifuge 13000g×10min at 4°C, discard the supernatant, wash the precipitate with 5% times with 75% ethanol, and dry it;
12. Add an appropriate amount of H2O or TE to dissolve the precipitate.
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