First, the working principle of the pipette
The pipette works on the principle that the piston moves through the telescopic movement of the spring to achieve aspiration and discharge. Pushed by the piston,
Part of the air is exhausted, and the liquid is sucked in at atmospheric pressure, and then the air is pushed out by the piston to discharge the liquid. When using a pipette,
It is easy to control the speed and strength of pipetting with the flexibility of the spring.
Second, the use of pipettes
1. Pipette specification operation steps
The first step is to set the pipetting volume.
When adjusting from large volume to small volume, it is a normal adjustment method. It can be rotated counterclockwise. When adjusting from small volume to large volume, it can be adjusted clockwise to the scale exceeding the set volume and then adjusted back to the set volume. Guarantee the best accuracy.
The second step is to assemble the pipette tip:
Insert the pipette tip vertically into the tip, and turn it slightly to the left and right.
Special note: It is very unacceptable to use the pipette to repeatedly hit the tip to tighten. For long-term operation, the parts in the pipette will be loose due to strong impact, and even the knob of the adjustment scale will be stuck. .
The third step is to aspirate and drain
Aspiration
↓
The tip of the tip needs to be immersed in the liquid surface below 3 mm
↓
Slowly suck and release, control the speed of the spring
↓
The tip of the tip leans against the inner wall of the container when draining
2. Pipetting tips
â— Pre-wetting aspiration
The viscous liquid can be accurately wetted by the pre-wetting of the tip. The sample is first aspirated and the liquid is sucked out. The inner wall of the tip absorbs a layer of liquid, so that the surface is saturated, then the sample is inhaled, and finally the liquid is discharged. The volume will be very accurate.
â— Positive aspiration and reverse aspiration
Positive aspiration refers to the normal method of aspirating. When operating, the aspirate can press the button to the first position to aspirate and release the button. Press the first gear first, and then discharge most of the liquid, then press the second gear to discharge the remaining liquid.
Reverse aspiration refers to pressing the button directly to the second gear and then releasing it, so that more liquid will be inhaled. Just press the first gear when the liquid is discharged. The multi-inhaled liquid can compensate for the surface adsorption inside the tip. The reverse aspiration is generally used in combination with the pre-wetting pipetting method, which is suitable for viscous liquids and volatile liquids.
Product Name: Junyi: Pipette | Bole Import Quality | New Design | Shanghai Spot
Product model: pipette
Product Exhibitor: Junyi Oriental
Product price: 300 yuan
Product specifications: product specification range (μl) increment (μl) 0.1～2.5 0.05 0.5～10 0.1 2～20 0.5 5～50 0.5 10～100 1 20～200 1 100～1000 5 1000～5000 50
Performance characteristics
• The ergonomic design of the palm is easy to control with full hands, reducing hand fatigue;
• Digital window to make the set range clear at a glance;
• Wide range of measurements (0.1-5000μl);
• Easy and quick calibration and repair using accessory tools;
• Quick and easy nozzle ejector;
• Removable nozzle fittings with high performance chemical corrosion resistance and high temperature autoclave.
Face Serum For Oily Skin,Face Serum For Dry Skin,Vitamin C Oil For Face,Face Serum For Women
Ningbo Roni Cosmetic Co,.Ltd , https://www.ronicosmetics.com