1. The smear method is a method of making the material evenly coated on the glass slide.
Smear materials include single-celled organisms, small algae, blood, bacterial culture fluids, loose tissues of animals and plants, testis, anthers, etc.
Please note when smearing: (1) The slide must be clean. (2) The slide should be flat. (3) The coating must be uniform. Smear the droplet to the right in the middle of the glass slide and apply evenly with a scalpel blade or toothpick. (4) The coating should be thin. Use another slide as a push slide, and gently push from the right to the left along the surface of the slide where the coating solution is dropped (the angle between the two slides should be 30 ° -45 °), coating it into a thin layer. (5) Fixed. If you need to fix it, you can fix it with chemical fixative or drying method (bacteria). (6) Dyeing. Methylene blue is used for bacteria, Wright's stain is used for blood, and iodine can sometimes be used. The staining solution should cover all the coated surfaces. (7) Rinse. Blot or dry with absorbent paper. (8) Sealing. Sealed with Canadian gum for long-term storage.
2. The compression method is a method of placing biological materials between a glass slide and a cover slip, applying a certain pressure, and compressing the tissue cells.
3. Mounting method is a method of integrating biological materials into glass slide specimens, which can be used to make temporary or permanent mounting slides.
The loading materials are: microscopic organisms such as Chlamydomonas, water sponge, amoeba, nematode; Hydra, leaf epidermis of plants; wings, feet, mouth organs of insects, human oral epithelial cells, etc.
Attention should be paid when making the slide method: (1) When holding the slide glass, it should be kept flat or placed on the platform. The amount of water should be appropriate when dripping, as it is just covered by the cover glass. (2) The material should be unfolded with dissecting needles or tweezers so as not to overlap and flatten on the same plane. (3) When placing the coverslip, slowly cover the water droplets from one side to prevent air bubbles. (4) When staining, place a drop of staining drop on one side of the cover slip, and draw it from the other side with absorbent paper to evenly stain the specimen under the cover slip. After coloring, use the same method, drop a drop of water, suck out the staining solution, and observe under a microscope.
4. A slice is a slide specimen made from a slice cut from a living body.
Due to different requirements, the blade can be used for free hand slicing, or the tissue block can be embedded in paraffin or collodion or frozen at low temperature, and sliced ​​with a microtome. Cut into 5-10 micron slices for optical microscope observation. Ultrathin sections cut with epoxy or methacrylic acid embedded tissue blocks, with a thickness of 20-50 nm, are specially designed for observation under an electron microscope. The slices of root tips and stems commonly used in teaching are generally called paraffin slices.
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