Matrix refers to the part other than the target analyte of the sample. Since the matrix component can significantly interfere with the analysis process and affect the accuracy of the analysis results, the industry collectively refers to these effects and interferences as matrix effects. This is a big problem to be avoided in the development and use of ELISA methods.
The reasons for the matrix effect are complex, including the design of the instrument, the composition of the reagents, the principle of the measurement method, the choice of quality control materials and other related factors. In the process of enzyme-linked immunoassay, how can we reduce the occurrence of matrix effects? Through continuous exploration experiments, our R & D personnel give the following related measures:
1. Be sure to use the diluent supplied with the kit. Unsuitable diluents will often increase the matrix effect and affect the immune reaction system, resulting in increased experimental errors;
2. Improve the design of the instrument;
3. Use appropriate sample processing methods, taking into account both the matrix effect and the extraction recovery factor;
4. Choose the correct method and methodological parameters.
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