This reagent is for research use only: Serum or plasma test principle: The LOX-1 kit is a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). Standards with known LOX-1 concentration and samples with unknown concentration are added to microwell The detection is carried out in the microplate. Human oxidized low-density lipoprotein receptor 1 ELISA detection kit first incubates LOX-1 and biotin-labeled antibody. After washing, HRP labeled with avidin was added. After incubation and washing, the unbound enzyme conjugate is removed, and then substrates A and B are added to act simultaneously with the enzyme conjugate. Produce colors. The color depth is proportional to the concentration of LOX-1 in the sample. Bring your own material distilled water. Sampler: 5ul, 10ul, 50ul, 100ul, 200, 500ul, 1000ul. Oscillator and magnetic stirrer etc. Safety Avoid direct contact with the stop solution and substrates A and B. Once exposed to these liquids, rinse with water as soon as possible. Do not eat, drink, smoke or use cosmetics during the experiment. Do not use your mouth to absorb any ingredients in the kit. Handling precautions Reagents should be stored according to label instructions, and returned to room temperature before use. The sparse standards should be discarded and cannot be stored. The slats not used in the experiment should be immediately returned to the packaging bag, sealed and stored to avoid deterioration. Unused other reagents should be packed or covered. Do not mix reagents of different batches. Use before warranty. Use a disposable pipette tip to avoid cross-contamination, and avoid using a sampler with a metal part when drawing the stop solution and substrates A and B. Use a clean plastic container to prepare the washing solution. Mix all components and samples in the kit thoroughly before use. When washing the enzyme-labeled plate, it should be fully patted dry. Do not put the absorbent paper directly into the enzyme-labeled reaction well to absorb water. Substrate A should evaporate to avoid opening the lid for a long time. Substrate B is sensitive to light and avoid prolonged exposure to light. Avoid contact with hands, it is toxic. The OD value should be read immediately after the experiment is completed. The order of adding reagents should be the same to ensure that all wells are incubated for the same time. Perform the incubation operation according to the time, the amount and order of the liquid indicated in the instructions. Sample collection, processing and storage methods Serum-avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins. After collecting blood, centrifuge at 1000 × g for 10 minutes to separate the serum and red blood cells quickly and carefully. Plasma ----- EDTA, citrate, heparin plasma can be used for detection. Centrifuge at 1000 × g for 30 minutes to remove particles. The cell supernatant was centrifuged at 1000 × g for 10 minutes to remove particles and polymers. Storage ------ If the sample is not used immediately, it should be divided into small parts and stored at -70 ℃ to avoid repeated freezing. If possible, do not use hemolysis or hyperlipidemia. If there are a large number of particles in the serum, centrifuge or filter before testing. Do not thaw at 37 ° C or higher. Thaw at room temperature and ensure that the sample is thawed evenly and adequately. Reagent preparation standards: Serial dilutions of standards should be prepared during the experiment and cannot be stored. Before dilution, the standard was mixed by shaking. Dilution of washing buffer (50 ×): 50-fold dilution with distilled water. Before use, mix all reagents thoroughly. Don't make the liquid generate a lot of foam, so as to avoid adding a large number of bubbles during sample addition, which will cause errors in sample addition. The number of slats required is determined by the number of samples to be tested plus the number of standard products. It is recommended to make multiple holes for each standard and blank hole. Each sample is determined according to its own quantity, and those that can use multiple holes can be used as much as possible. Add 50ul of the diluted standard to the reaction well and 50ul of the sample to be tested in the reaction well. Immediately add 50ul of biotin-labeled antibody. Cover the membrane plate, gently shake and mix, and incubate at 37 ° C for 1 hour. Shake off the liquid in the wells, fill each well with washing liquid, shake for 30 seconds, shake off the washing liquid, pat dry with absorbent paper. Repeat this operation 3 times. If washing with a plate washer, the number of washes is increased once. Add 80 ul of streptavidin-HRP to each well, gently shake and mix, and incubate at 37 ° C for 30 minutes. Shake off the liquid in the wells, fill each well with washing liquid, shake for 30 seconds, shake off the washing liquid, pat dry with absorbent paper. Repeat this operation 3 times. If washing with a plate washer, the number of washes is increased once. Add 50ul of substrate A and B to each well, mix gently by shaking, and incubate at 37 ° C for 10 minutes. Avoid light. Remove the enzyme labeling plate and quickly add 50ul of stop solution. After adding the stop solution, the results should be measured immediately. The OD value of each well was measured at a wavelength of 450 nm. The results above the No. 6 standard are non-linear, and accurate results cannot be obtained based on this standard curve. Kit performance 1. Sensitivity: The minimum detection concentration is less than No. 1 standard. Linearity of dilution. The coefficient R of the linear regression of the sample and the expected concentration is 0.990. 2. Specificity: Does not react with other human cytokines. 3. Repeatability: The coefficients of variation within and between plates are less than 10%. Judgment and analysis of results 1. Instrument value: read the OD value of each well on a microplate reader with a wavelength of 450 nm. 2. Take the absorbance OD value as the ordinate (Y), and the corresponding LOX-1 standard concentration as the abscissa (X ), The corresponding curve is made, and the LOX-1 content of the sample can be converted from the standard curve to the corresponding concentration according to its OD value. 3. Detection value range: 0-8.0IU / ml 4. Sensitivity: 0.01 IU / ml Anti-ZAP-70 / FITC fluorescein-labeled zeta-related protein 70 antibody IgG Y74338 0.2ml Anti-ZCWCC1 / FITC fluorescein-labeled ZCWCC1 antibody IgG Y74339 0.2 mlAnti-Z-DEVD-FMK / FITC fluorescein-labeled CASPASE3 apoptosis inhibitor IgG Y74340 0.2mlAnti-Zfp91 / FITC fluorescein-labeled leukemia-related new gene antibody IgG Y74341 0.2mlAnti-ZNF217 / FITC fluorescein-labeled zinc finger lipoprotein 217 antibody IgG Y74342 0.2mlAnti-ZNF268 (1a) / FITC fluorescein-labeled zinc finger lipoprotein-1a antibody IgG Y74343 0.2mlAnti-ZNF268 (1b) / FITC fluorescein-labeled zinc finger lipoprotein-1b antibody IgG Y74344 0.2mlAnti-ZNF268 (1c ) / FITC fluorescein-labeled zinc finger lipoprotein-1c antibody IgG Y74345 0.2mlAnti-ZNF300 / FITC fluorescein-labeled zinc finger protein 300 antibody IgG Y74346 0.2mlAnti-ZNF307 / FITC fluorescein-labeled zinc finger protein 307 antibody IgG Y74347 0.2mlAnti- ZNF474 / FITC fluorescein-labeled zinc finger protein 474 antibody IgG Y74348 0.2mlAnti-ZO-1 / FITC fluorescein-labeled cytoplasmic tight adhesion protein 1 antibody IgG Y74349 0.2ml Anti-ZW10 peptide / FITC fluorescein-labeled centromere antibody IgG Y74350 0. 2mlAnti-Zyxin / FITC fluorescein-labeled zonulin antibody IgG Y74351 0.2mlAnti-Phospho-Zyxin (Ser142 / Ser143) / FITC fluorescein-labeled phosphorylated spectrin protein antibody IgG Y74352 0.2mlAnti-TORC1 / CRTC1 / FITC fluorescein-labeled ring Adenylate response element binding protein transcription coactivator TORC1 antibody IgG Y74353 0.2ml Anti-Torc2 / Crtc2 / FITC fluorescein-labeled CREB transcription coactivator TORC2 antibody IgG Y74354 0.2ml Anti-Phospho-Torc2 / Crtc2 (Ser171) / FITC fluorescein Labeled phosphorylated CREB transcription coactivator TORC2 antibody IgG Y74355 0.2ml Anti-Gentamicin Monoclonal Antibody / Gold colloidal gold ion-labeled mouse anti-gentamicin monoclonal antibody IgG Y74356 0.5ml (35nm-40nm) Anti-Gentamicin / Gold colloidal gold Ion-labeled rabbit gentamicin antibody IgG Y74357 0.5ml (35nm-40nm)
It is a simple Climbing Machine and can be folded when
you are rest . It can`t be occupy much space . But it is hot sell in some
countries .For example , Korea , Japan , EU , Chinese . Etc .Also the price is
cheap , but the Fitness Equipment machine use easily . It also called vertical
climber cardio exercise machine . It don`t has display screen watches and
suitable for one people to do sports on it . The gross weight is only 22KGS and
one small 20`FT container can load 350PCS , It can save your much shipment
charge . Because it is smaller and light . Also the price is low .
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XJ-CM-04 |
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SPECIFICATION |
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G.W |
22KGS |
N.W |
20KGS |
Max Weight |
150KGS |
Meas |
165*30.8*15CM |
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Max Height |
200CM |
Size |
68*100*200CM |
QTY |
1PC/CN |
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Ctn |
350PCS/20`FT |
730PCS/40`GP |
810PCS /40`HQ |
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Folding Climbing Machine,Vertical Climber Climbing Machine,Vertical Climber Cardio Exercise Machine,Vertical Climber Machine
ZHEJIANG MEIER FITNESS EQUIPMENT CO., LTD , http://www.chinameier.com